This kit is a full-component lyophilized fluorescent PCR reagent for patients with suspected cases of pneumonia, suspected aggregation cases of COVID-19 infection, and other patients who need to undergo clinical diagnosis of new coronavirus infection.
This kit is a full-component lyophilized fluorescent PCR reagent for patients with suspected cases of pneumonia, suspected aggregation cases of COVID-19 infection, and other patients who need to undergo clinical diagnosis of new coronavirus infection. The COVID-19 ORF1ab and N genes were specifically tested.
The COVID-19 nucleic acid detection kit is based on reverse transcription PCR combined with fluorescent probe amplification detection technology. Fluorescent labeling of specific fluorescent probes. 5'is labeled with a fluorescent reporter group and 3'is labeled with a fluorescent quencher group. Before amplification, the fluorescent signal of the reporter group is absorbed by the quencher group and cannot excite the fluorescent signal; during the PCR amplification process, Taq polymerase The 5'-3' exonuclease activity cuts off the fluorescent group of the probe, separates the fluorescent reporter group and the fluorescent quencher group, enables the fluorescence monitoring system to receive the fluorescence signal, and records the increase of the fluorescence level by the fluorescence PCR instrument .
The Table1：COVID-19 Real-Time Fluorescence PCR Detection Kit (lyophilized)
Component | Amount |
RT-PCR Mix | 1 bottle |
Positive control (lyophilized powder) | 1 tube |
Negative control | 1 tube |
Manual | 1 |
Note: Note: Components to be prepared by the customer and not provided in this kit: RNA nucleic acid extraction kit
The kit is stored at 25 ±5℃ before use, and the validity period is 24 months.
Suggestion: After adding nuclease-free water to dissolve the RT-PCR Mix, the reaction solution should be divided into PCR reaction tubes according to a single portion of the reaction system and stored at -20℃ to avoid repeated freezing and thawing of the reagents and affecting the quality of the reagents.
